ANTONIO MACHADO

  • Profesor de Microbiología Patógena e Inmunología
  • Colegio de Ciencias Biológicas y Ambientales, COCIBA
  • 297-1700 ext 1426
  • amachado@usfq.edu.ec

ESTUDIOS

  • Ingeniería biomédica, Universidad de Minho

EXPERIENCIA LABORAL

Investigador del grupo de investigación en Biofilms Bacterianos, Universidad de Minho
( 2009 - 2014 )
Responsable por una área de investigación solo suya y de ser tutor de varias áreas de investigación de estudiantes de maestría y de doctorado.
Instrutor del Workshop Biolfim Transcriptomics, Universidad de Minho
( 2010 - 2012 )
Enseñanza de la tecnología de la transcripción de RNA en Biofilms Bacterianos a través de Real Time PCR.
Investigador extranjero, Vitginia Commonwealth University
( 2011 - 2012 )
Investigador extranjero en el Departamento de Inmunología del Instituto de Investigación de la Universidad (VCU-Virginia Commonwealth University).
Profesor de Microbiología y Inmunología, Escuela Politécnica de Infermeria de la Cruz Roja
( 2007 - 2010 )
Profesor de Microbiología y Inmunología para la carrera de Infermeria.
Investigador del grupo de investigación en Biología Celular y Molecular, Universidad de Minho
( 2008 - 2008 )
Joven Investigador de un grupo de investigación de bioinformática especializada en drug design.
Técnico de laboratorio de analisis clínicas, Hospital San Miguel
( 2007 - 2007 )
Técnico de laboratorio de análisis clínicas en el servicio de patología clínica en el Hospital San Miguel

PUBLICACIONES

Determination of the Microbial and Chemical Loads in Rivers from the Quito Capital Province of Ecuador (Pichincha) – A Preliminary Analysis of Microbial and Chemical Quality of the Main Rivers
Vaginal Microbiota Evaluation and Lactobacilli Quantification by qPCR in pregnant and non pregnant Women: a pilot study - Aceptado luego de revisión en Frontiers in celular and infection microbiology - IN PRESS
Influence of temperature on biofilm formation by Listeria innocua
Bacterial identification of the vaginal microbiota in Ecuadorian pregnant teenagers: an exploratory analysis.
Estudio Preliminar: Caracterización y cuantificación molecular de la microbiota vaginal en mujeres ecuatorianas en edad reprodutiva
Evaluación de la carga microbiana y química en ríos de varias provincias del Ecuador
Evaluation of the microbial and chemical load in rivers from the province of Pichincha in Ecuador
Symptomatic and Asymptomatic Vaginitis in Ecuadorian Women: An epidemiologic analysis
Multiplex Peptide Nucleic Acid Fluorescence In Situ Hybridization (PNA-FISH) for Diagnosis of Bacterial Vaginosis
Deteccion de Helicobacter sp. resritente a la claritromicina mediante florecencia de hibridacion in situ
Diagnosis of bacterial vaginosis by a new multiplex peptide nucleic acid fluorescence in situ hybridization method
Influence of Biofilm Formation by Gardnerella vaginalis and Other Anaerobes on Bacterial Vaginosis.
Can a specific sub-group of biofilm- forming Gardnerella vaginalis strains be the real causative agent of bacterial vaginosis?
Fluorescence In Situ Hybridization Method Using a Peptide Nucleic Acid Probe for Identification of Lactobacillus spp. in Milk Samples.
Fluorescence In Situ Hybridization Method Using Peptide Nucleic Acid Probes for rapid detection of Lactobacillus and Gardnerella spp.
Gardnerela vaginalis and other anaerobes commonly associated with bacterial vaginosis.
Interactions between Lactobacillus crispatus and Bacterial Vaginosis (BV)-Associated Bacterial Species in Initial Attachment and Biofilm Formation
Lactobacilli and its metabolites as potential probiotics against Gardnerella vaginalis.
Quantitative analysis of initial adhesion of Bacterial vaginosis anaerobes in ME-180 cells
Quantitative analysis of initial adhesion of bacterial vaginosis anaerobes in ME-180 cells.
Reciprocal interference between Lactobacillus spp. and Gardnerella vaginalis on initial adherence to epithelial cells.
10. O papel dos biofilmes na vaginose bacteriana
29. Methods in vitro of initial adhesion
Competitive initial adhesion between Lactobacillus spp. and Gardnerella vaginalis strains against vaginal epithelium.
Detection of Lactobacillus and Gardnerella species in vaginal samples by PNA-FISH.
In silico vs in vitro analysis of primer specificity for the detection of Gardnerella vaginalis, Atopobium vaginae and Lactobacillus spp.
Social interaction by BV anaerobes in initial adhesion and biofilm assays.
Comparison between classical and molecular (FISH and PCR) methods for Lactobacillus spp. detection in Clinical Samples.
Gardnerella vaginalis: the role of biofilms in the pathogenesis of bacterial vaginosis
Lactobacillus spp. Identification in Mixed Samples by PNA FISH.
Presence of Gardnerella vaginalis in healthy portuguese women - a pilot study.
Application of Desirability-Based Multi(bi)-objective Optimization in the design of selective arylpiperazine derivates for the 5-HT1A serotonin receptor.
Fractal protein structure revisited: Topological, kinetic and thermodynamic relationships.
Influence of arylpiperazines aromatic structure over differential affinity for 5-HT1A and 5-HT2A receptors.
QSAR study in serotonin receptors and piperazine’s selectivity

INTERESES

Doctorado en Ingeniería Biomédica , Toxicología Análitica Clínica y Forense, Diagnóstico de Patógenos, Desarrollo de Metodología y Virulencia de Bacterias